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Cellular/Cell-Mediated/T-Cell Immunity

August 30, 2023
by Santhakumar Raja
3 min read
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Cell-mediated immunity is an immune response that does not involve antibodies or complement, but rather involves the activation of macrophages, natural killer cells, antigen-specific cytotoxic T-lymphocytes, and the release of...

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  • Health Science

Application of rDNA Technology and Genetic Engineering the Production of Insulin

August 30, 2023
by Santhakumar Raja
2 min read
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Human insulin was the first recombinant-derived product. It is used for the treatment of diabetes. Fredrick Sanger and his co-worker identified the insulin structure stating that it is made up of two polypeptide chains of A and B...

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  • Health Science

Application of rDNA Technology and Genetic Engineering in the Production of Interferon

August 29, 2023
by Santhakumar Raja
5 min read
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Interferons are low molecular weight proteins produced by virus infected cells, which in non-infected cells can induce the formation of a second protein. This protein has the ability to prevent transcription of any viral mRNA...

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  • Health Science

Applications of rDNA Technology and Genetic Engineering in Medicine

August 28, 2023
by Santhakumar Raja
3 min read
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Genetic engineering has an important role to play in the production of medicines. Microorganisms and plant-based substances are used for producing various drugs, vaccines, enzymes, and hormones at low costs. Genetic engineering...

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  • Health Science

Techniques Used in Recombinant DNA Technology

August 28, 2023
by Santhakumar Raja
5 min read
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In recombinant DNA technology, many different techniques are used to achieve different requirements or to obtain proper information so that an exact inference can be drawn out during genetic engineering. Gel electrophoresis...

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  • Health Science

Expression of Cloned Gene Within the Host

August 27, 2023
by Santhakumar Raja
2 min read
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To express a cloned gene (for a desired product) in a transformed cell means that it has to transcript and translate. Sometimes functional proteins are produced by post-translation modifications. Protein synthesis is based on the...

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  • Health Science

Hybrid Released Translation (HRT) Method

August 27, 2023
by Santhakumar Raja
2 min read
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The HRT method is utilised for recombinants without introns. The most difficult step in applying hybridisation is obtaining a suitable probe. If the target DNA is represented with highly abundant mRNA, a small number of cDNA...

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  • Health Science

Colony Hybridisation (or Nucleic Acid Hybridisation) by Replica Plate Method

August 27, 2023
by Santhakumar Raja
1 min read
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The replica plate method is used in genetic engineering for identifying transformed bacterial cells (cells containing foreign DNA). Only some of the cells transformed with a specific DNA may actually have foreign DNA. Thus, it is...

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An Expression Vector
  • Health Science

Vectors for Preparing Single Stranded DNA

August 27, 2023
by Santhakumar Raja
4 min read
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For conventional DNA sequencing templates of signal stranded DNAs are used. PCR-based methods can be employed to manufacture them. Also vectors based on certain bacteriophages whose genomes assume a single stranded DNA at some...

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Bacterial Artificial Chromosome (BAC) Vectors
  • Health Science

Vectors for Cloning Larger DNA Fragments

August 26, 2023
by Santhakumar Raja
4 min read
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Human and other mammalian genomes are very large, therefore, vectors are needed to clone large DNA fragments. Many vectors have been prepared that aid in the characterisation and expression of large genes or gene complexes. These...

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Recent Posts

  • Cellular/Cell-Mediated/T-Cell Immunity
  • Application of rDNA Technology and Genetic Engineering the Production of Insulin
  • Application of rDNA Technology and Genetic Engineering in the Production of Interferon
  • Applications of rDNA Technology and Genetic Engineering in Medicine
  • Techniques Used in Recombinant DNA Technology
  • Expression of Cloned Gene Within the Host
  • Hybrid Released Translation (HRT) Method
  • Colony Hybridisation (or Nucleic Acid Hybridisation) by Replica Plate Method
  • Vectors for Preparing Single Stranded DNA
  • Vectors for Cloning Larger DNA Fragments
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