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Growth of Animal Cells in Culture

March 30, 2023
by Santhakumar Raja
4 min read
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Animal cell culturing is more difficult than microbial culturing because animal cells demand more nutrients and should be attached to specially coated surfaces for their growth. Still there are various undifferentiated and...

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Evaluation of Microbial Stability of Formulations
  • Health Science

Evaluation of Microbial Stability of Formulations

March 29, 2023
by Santhakumar Raja
6 min read
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Microbial stability testing involves the following five steps: Sampling : The number of containers to be used and volume to be tested are specified in the Pharmacopoeia. Samples of aseptically manufactured product are collected...

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Preservation of Pharmaceutical Products Using Antimicrobial Agents
  • Health Science

Preservation of Pharmaceutical Products Using Antimicrobial Agents

March 27, 2023
by Santhakumar Raja
3 min read
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Antimicrobial preservatives are added to non-sterile dosage forms to protect them from microbial growth or from microorganisms that are introduced inadvertently during or after the manufacturing process. Antimicrobial...

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Assessment of Microbial Contamination and Spoilage
  • Health Science

Assessment of Microbial Contamination and Spoilage

March 25, 2023
by Santhakumar Raja
2 min read
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Microbial contamination and spoilage are assessed by the following methods: Physical and Chemical Changes: Microbial contamination and spoilage in various pharmaceutical formulations can be identified by the physical and...

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Standardisation of Amino Acids
  • Health Science

Standardisation of Amino Acids – theteche.com

March 21, 2023
by Santhakumar Raja
2 min read
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Microbiological assays were used for quantitation of amino acids since many years as they were the most reliable, and specific available tests. They can be used for any type of biological material; however sometimes they are...

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Cavities Prepared in Sterile Agar Medium for Assay of Antibiotics
  • Health Science

Cup-Plate or Cylinder-Plate Method

March 18, 2023
by Santhakumar Raja
4 min read
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This method relies on the diffusion of an antibiotic from a vertical cavity or a cylinder 2 through the solidified agar layer in a petri plate. The growth of test microorganisms is observed to be inhibited in a circular area or...

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Microbiological Assay by Tube Assay Method
  • Health Science

Turbidimetric or Tube Assay Method

March 18, 2023
by Santhakumar Raja
2 min read
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This method depends on the microbial culture growth in a uniform solution of antibiotic in a fluid medium favourable to its rapid growth in the absence of antibiotic. This method involves a shorter incubation period (4-5 hours)...

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Curve of Diameter of Zone of Inhibition versus Log Concentration of Antibiotics
  • Health Science

Methods for Standardisation of Antibiotics

March 18, 2023
by Santhakumar Raja
3 min read
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Antibiotics are the agents that kill or reduce the bacterial growth. They belong to a class of antimicrobials (a larger group also comprising of anti-viral, anti-fungal, and anti- . parasitic drugs). Antibiotics are chemicals...

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Methods of Prevention of Contamination
  • Health Science

Methods of Prevention of Contamination – theteche.com

March 16, 2023
by Santhakumar Raja
4 min read
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The steps by which microbial contamination of products can be prevented in aseptic areas are discussed below: Personnel : They can take the following measures: Access to production areas by the unauthorised personnel should be...

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Different Sources of Contamination in an Aseptic Area
  • Health Science

Different Sources of Contamination in an Aseptic Area

March 16, 2023
by Santhakumar Raja
3 min read
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There are various contamination sources in an aseptic area, which are discussed below: Personnel : Those supervising, performing, or controlling drug manufacturing are a potential source of microbial contamination, due to the...

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Recent Posts

  • Growth of Animal Cells in Culture
  • Evaluation of Microbial Stability of Formulations
  • Preservation of Pharmaceutical Products Using Antimicrobial Agents
  • Assessment of Microbial Contamination and Spoilage
  • Standardisation of Amino Acids – theteche.com
  • Cup-Plate or Cylinder-Plate Method
  • Turbidimetric or Tube Assay Method
  • Methods for Standardisation of Antibiotics

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Featured Posts

  • Growth of Animal Cells in Culture
    by Santhakumar Raja
  • Evaluation of Microbial Stability of Formulations Evaluation of Microbial Stability of Formulations
    by Santhakumar Raja
  • Preservation of Pharmaceutical Products Using Antimicrobial Agents Preservation of Pharmaceutical Products Using Antimicrobial Agents
    by Santhakumar Raja
  • Assessment of Microbial Contamination and Spoilage Assessment of Microbial Contamination and Spoilage
    by Santhakumar Raja
  • Standardisation of Amino Acids Standardisation of Amino Acids – theteche.com
    by Santhakumar Raja
  • Cavities Prepared in Sterile Agar Medium for Assay of Antibiotics Cup-Plate or Cylinder-Plate Method
    by Santhakumar Raja
  • Microbiological Assay by Tube Assay Method Turbidimetric or Tube Assay Method
    by Santhakumar Raja
  • Curve of Diameter of Zone of Inhibition versus Log Concentration of Antibiotics Methods for Standardisation of Antibiotics
    by Santhakumar Raja
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