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Category - Health Science

pBR322 Vector Plasmid
  • Health Science

Study of Cloning Vectors

August 26, 2023
by Santhakumar Raja
4 min read
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Cloning vector is a DNA molecule in which when a foreign DNA is integrated achicves the capability to replicate it within itself to produce many clones of recombinant DNA. Characteristics of a Cloning Vector Its size should be...

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  • Health Science

Insertion of Target DNA Into a Vector

August 25, 2023
by Santhakumar Raja
3 min read
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The target DNA or the DNA insert isolated and enzymatically cleaved by the selective restriction endonuclease, are ligated (joined) through the action of ligase enzyme to the vector DNA. As a result, an rDNA molecule known as...

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Basic Steps of Recombinant DNA Technology Using the Bacterial Plasmid as Cloning Vector
  • Health Science

Introduction for Recombinant DNA Technology

August 24, 2023
by Santhakumar Raja
1 min read
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In recombinant DNA (rDNA/rec DNA) technology, DNA molecules from two different sources are joined and inserted into a host organism to get products useful for human use (figure). A clone refers to a group of cells or organisms...

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  • Health Science

Protein Engineering

August 21, 2023
by Santhakumar Raja
2 min read
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The process of developing proteins having desired functions by manipulating their stability and specificity is termed protein engineering. This can be done by taking two main approaches, rational design and irrational design (or...

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Manufacturing RNA Using GMOs
  • Health Science

Genetic Engineering

August 20, 2023
by Santhakumar Raja
5 min read
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Genetic engineering involves deliberate DNA manipulation in organisms to alter their genes. Although the organisms whose genes are being altered may not be microbes, but the substances and techniques involved are obtained from...

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  • Health Science

Increasing the Stability and Biological Activity of Proteins

August 19, 2023
by Santhakumar Raja
7 min read
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The industrial applications or therapeutic uses of enzymes/proteins can be appropriately brought into use by increasing their half-lives or thermostability. Proteins with enhanced stability can be obtained by the following...

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Sachse Mohrs Theory
  • Health Science

Sachse Mohr’s Theory (Theory of Strainless Rings)

August 18, 2023
by Santhakumar Raja
5 min read
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Baeyer’s strain theory successfully explained the relative stability of cyclic compounds having 6-membered rings (or more appropriately having 5-membered rings), but it failed to explain the stability of higher compounds...

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  • Health Science

Classification of Fatty Acids

August 18, 2023
by Santhakumar Raja
3 min read
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A carboxylic acid with a long, unbranched, saturated or unsaturated aliphatic tail (or chain) is called a fatty acid. Natural fatty acids found abundantly carry carbon atoms in even number because they are biosynthesised using...

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Effect of Substituents on Acidity
  • Health Science

Effect of Substituents on Acidity

August 17, 2023
by Santhakumar Raja
2 min read
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If electron-withdrawing groups are present on the benzene ring of benzoic acid, the benzoate ion stabilises and acidity thus increases. This is attributed to inductive and resonance effects. In case of p-nitrobenzoic acid and...

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Basicity substituted amines
  • Health Science

Effect of Substituents on Basicity

August 17, 2023
by Santhakumar Raja
2 min read
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Presence of electron releasing groups on the benzene ring of aromatic amines enhances the electron density on nitrogen, thus increase the basicity. On the contrary, presence of electron withdrawing groups reduces the basicity of...

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Recent Posts

  • Cellular/Cell-Mediated/T-Cell Immunity
  • Application of rDNA Technology and Genetic Engineering the Production of Insulin
  • Application of rDNA Technology and Genetic Engineering in the Production of Interferon
  • Applications of rDNA Technology and Genetic Engineering in Medicine
  • Techniques Used in Recombinant DNA Technology
  • Expression of Cloned Gene Within the Host
  • Hybrid Released Translation (HRT) Method
  • Colony Hybridisation (or Nucleic Acid Hybridisation) by Replica Plate Method

Featured Posts

  • Cellular/Cell-Mediated/T-Cell Immunity
    by Santhakumar Raja
  • Application of rDNA Technology and Genetic Engineering the Production of Insulin
    by Santhakumar Raja
  • Application of rDNA Technology and Genetic Engineering in the Production of Interferon
    by Santhakumar Raja
  • Applications of rDNA Technology and Genetic Engineering in Medicine
    by Santhakumar Raja
  • Techniques Used in Recombinant DNA Technology
    by Santhakumar Raja
  • Expression of Cloned Gene Within the Host
    by Santhakumar Raja
  • Hybrid Released Translation (HRT) Method
    by Santhakumar Raja
  • Colony Hybridisation (or Nucleic Acid Hybridisation) by Replica Plate Method
    by Santhakumar Raja
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