Colony Hybridisation (or Nucleic Acid Hybridisation) by Replica Plate Method

The replica plate method is used in genetic engineering for identifying transformed bacterial cells (cells containing foreign DNA). Only some of the cells transformed with a specific DNA may actually have foreign DNA. Thus, it is essential to screen those cells having foreign DNA by the technique of colony hybridisation (only for bacterial cells) figure. A similar technique named plaque hybridisation is used for screening transformed bacteriophages.

Steps in Colony Hybridisation Technique

The principle of colony hybridisation involves in-situ hybridisation of transformed bacterial cells with a radioactive probe sequence. Due to the specificity of probe, a single colony can be rapidly identified (through radioactivity) amongst thousand of colonies.

Read More Topics
Vectors for preparing single stranded DNA
Vectors for cloning larger DNA fragments
Insertion of target DNA into a vector

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Santhakumar Raja

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